This lesson continues with the second workshop on reproducible science, focusing on additional open source tools for researchers and data scientists, such as the R programming language for data science, as well as associated tools like RStudio and R Markdown. Additionally, users are introduced to Python and iPython notebooks, Google Colab, and are given hands-on tutorials on how to create a Binder environment, as well as various containers in Docker and Singularity.

This talk goes over Neurobagel, an open-source platform developed for improved dataset sharing and searching. 

In this lesson, you will learn about the BRAIN Initiative Cell Atlas Network (BICAN) and how this project adopts a federated approach to data sharing. 

In this second part of the lecture Data Science and Reproducibility, you will learn how to apply the awareness of the intersection between neuroscience and data science (discussed in part one) to an understanding of the current reproducibility crisis in biomedical science and neuroscience. 

This lecture covers the benefits and difficulties involved when re-using open datasets, and how metadata is important to the process.

This lesson provides a quick tour of some data repositories and how to download and manipulate data from them.

In this lesson, attendees will learn about the data structure standards, specifically the Brain Imaging Data Structure (BIDS), an INCF-endorsed standard for organizing, annotating, and describing data collected during neuroimaging experiments. 

This lecture contains an overview of electrophysiology data reuse within the EBRAINS ecosystem.

This lecture contains an overview of the Distributed Archives for Neurophysiology Data Integration (DANDI) archive, its ties to FAIR and open-source, integrations with other programs, and upcoming features.

This lecture explains the concept of federated analysis in the context of medical data, associated challenges. The lecture also presents an example of hospital federations via the Medical Informatics Platform.

This lesson contains both a lecture and a tutorial component. The lecture (0:00-20:03 of YouTube video) discusses both the need for intersectional approaches in healthcare as well as the impact of neglecting intersectionality in patient populations. The lecture is followed by a practical tutorial in both Python and R on how to assess intersectional bias in datasets. Links to relevant code and data are found below. 

This is a hands-on tutorial on PLINK, the open source whole genome association analysis toolset. The aims of this tutorial are to teach users how to perform basic quality control on genetic datasets, as well as to identify and understand GWAS summary statistics. 

This is a tutorial on using the open-source software PRSice to calculate a set of polygenic risk scores (PRS) for a study sample. Users will also learn how to read PRS into R, visualize distributions, and perform basic association analyses. 

This lesson is an overview of transcriptomics, from fundamental concepts of the central dogma and RNA sequencing at the single-cell level, to how genetic expression underlies diversity in cell phenotypes. 

This is a tutorial introducing participants to the basics of RNA-sequencing data and how to analyze its features using Seurat. 

This tutorial demonstrates how to perform cell-type deconvolution in order to estimate how proportions of cell-types in the brain change in response to various conditions. While these techniques may be useful in addressing a wide range of scientific questions, this tutorial will focus on the cellular changes associated with major depression (MDD). 

This is a tutorial on how to simulate neuronal spiking in brain microcircuit models, as well as how to analyze, plot, and visualize the corresponding data. 

Maximize Your Research With Cloud Workspaces is a talk aimed at researchers who are looking for innovative ways to set up and execute their life science data analyses in a collaborative, extensible, open-source cloud environment. This panel discussion is brought to you by MetaCell and scientists from leading universities who share their experiences of advanced analysis and collaborative learning through the Cloud.

This talk describes the relevance and power of using brain atlases as part of one's data integration pipeline.