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Fundamental Methods for Single-Cell Transcriptome Analysis

This course, consisting of one lecture and two workshops, is presented by the Computational Genomics Lab at the Centre for Addiction and Mental Health and University of Toronto. The lecture deals with single-cell and bulk level transciptomics, while the two hands-on workshops introduce users to transcriptomic data types (e.g., RNAseq) and how to perform analyses in specific use cases (e.g., cellular changes in major depression). 

Course Features
Lecture
Tutorials
Video
Slides
Code / Datasets
Lessons of this Course
1
1
Duration:
1:29:08

This lesson is an overview of transcriptomics, from fundamental concepts of the central dogma and RNA sequencing at the single-cell level, to how genetic expression underlies diversity in cell phenotypes. 

2
2
Duration:
1:19:17
Speaker:

This is a tutorial introducing participants to the basics of RNA-sequencing data and how to analyze its features using Seurat. 

3
3
Duration:
1:15:14
Speaker:

This tutorial demonstrates how to perform cell-type deconvolution in order to estimate how proportions of cell-types in the brain change in response to various conditions. While these techniques may be useful in addressing a wide range of scientific questions, this tutorial will focus on the cellular changes associated with major depression (MDD).