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Introduction to Transcriptomic Data Types

Difficulty level

This is a tutorial introducing participants to the basics of RNA-sequencing data and how to analyze its features using Seurat. 

Topics covered in this lesson
  • Be introduced to the concept of "reads" and "counts"
  • Import and normalize a counts matrix
  • Perform dimensionality reduction
  • Visualize clusters of cells
  • Visualize gene expression across cells
  • Identify cell type-specific marker genes (i.e., differentially expressed genes (DEGs))
Technology requirement