Introduction to Transcriptomic Data Types
Introduction to Transcriptomic Data Types
This is a tutorial introducing participants to the basics of RNA-sequencing data and how to analyze its features using Seurat.
Topics covered in this lesson
- Be introduced to the concept of "reads" and "counts"
- Import and normalize a counts matrix
- Perform dimensionality reduction
- Visualize clusters of cells
- Visualize gene expression across cells
- Identify cell type-specific marker genes (i.e., differentially expressed genes (DEGs))
Prerequisites
Technology requirement
- Git, GitHub
- Seurat R Toolkit for single cell genomics
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