A virtual workshop with lectures and hands-on tutorials that will teach participants how to use open-source Miniscopes for in vivo calcium imaging. This workshop is designed to introduce all aspects of using Miniscopes, including basic principles of Miniscope design and imaging, how to build and attach a Miniscope, how to implant a GRIN lens for imaging deep structures, and how to analyze imaging data. The workshop will also cover the most recent developments in Miniscope technology and highlight some of the best advances in this exciting and growing field.
2021 Virtual Miniscope Workshop
Course Features
Videos
Lectures
Tutorials
Lessons of this Course
1
1
Duration:
16:23
This introductory lesson welcomes users to the virtual learning series, explaining some of the background behind open-source miniscopes, as well as outlining the rest of the lessons in this course.
2
2
Duration:
42:16
Speaker:
This lesson provides an overview of the Miniscope project, explaining the motivation behind the how and why of Miniscope development, why Miniscopes may be useful for researchers, and the differences between previous and current versions.
3
3
Duration:
1:00:40
Speaker:
This lesson will go through the theory and practical techniques for implanting a GRIN lens for imaging in mice.
4
4
Duration:
1:04:28
This lesson provides instruction on how to build a Miniscope and stream data, including an overview of the software involved.
5
5
Duration:
00:53:34
Best practices: the tips and tricks on how to get your Miniscope to work and how to get your experiments off the ground.
6
6
Duration:
00:01:59
Speaker:
Overview of the content for Day 1 of this course.
8
8
Duration:
00:24:03
This talk compares various sensors and resolutions for in vivo neural recordings.
9
9
Duration:
00:21:51
This talk delves into challenges and opportunities of Miniscope design, seeking the optimal balance between scale and function.
10
10
Duration:
00:17:56
Attendees of this talk will learn aobut computational imaging systems and associated pipelines, as well as open-source software solutions supporting miniscope use.
11
11
Duration:
00:21:06
This talk covers the present state and future directions of calcium imaging data analysis, particularly in the context of one-photon vs two-photon approaches.
12
12
Duration:
00:19:43
In this talk, results from rodent experimentation using in vivo imaging are presented, demonstrating how the monitoring of neural ensembles may reveal patterns of learning during spatial tasks.
13
13
Duration:
00:57:26
Speaker:
How to start processing the raw imaging data generated with a Miniscope, including developing a usable pipeline and demoing the Minion pipeline.
14
14
Duration:
00:49:16
The direction of miniature microscopes, including both MetaCell and other groups.
15
15
Duration:
00:11:01
Speaker:
Overview of the content for Day 2 of this course.
16
16
Duration:
01:28:03
Speaker:
This hands-on tutorial explains how to run your own Minion session in the MetaCell cloud using jupityr notebooks.
17
17
Duration:
00:20:34
Speaker:
In this hands-on analysis tutorial, users will mimic a kernel crash and learn the steps to restore inputs in such a case.
18
18
Duration:
01:49:40
Speaker:
This lesson will go through how to extract cells from video that has been cleaned of background noise and motion.
19
19
Duration:
00:27:23
Speaker:
This final hands-on analysis tutorial walks users through the last visualization steps in the cellular data.
20
20
Duration:
00:04:56
Speaker:
Summary and closing remarks for this three-day course.
