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In-vivo imaging & behavior

Difficulty level

Best practices: the tips and tricks on how to get your Miniscope to work and how to get your experiments off the ground.

Topics covered in this lesson
  • Experimental timeline
  • Handling animal subjects
  • Attaching the Miniscope
  • Setting exposure and focus
  • Habituating the animal subject to the scope
  • Removing the scope
  • Behavioural videos
  • FAQs
    • Triggering miniscope acquisition from an external source
    • Finding the optimal field of view
    • Capturing the same field of view
    • Commutators
    • Coaxial cables
    • Aligning imaging with behaviors
  • Tracking behavior for Miniscope recordings
  • Open-source behavior software
  • ezTrack
  • ezTrack notebook example
  • Wrap-up and questions