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Surgical Techniques: GRIN Lens & Baseplate Surgery

Difficulty level

This lesson will go through the theory and practical techniques for implanting a GRIN lens for imaging in mice.

Topics covered in this lesson
  • Implanted GRIN lens: V3 vs V4
  • Surgery and implantation
    • Virus injection
    • Implant lens
    • Cement baseplate
  • Overview of implant surgery
    • Skull preparation
    • Craniotomy and aspiration of cortex
    • Insert and secure lens
    • Protect lens
  • Overview of baseplating