Minian: An Open-Source Miniscope Analysis Pipeline
Minian: An Open-Source Miniscope Analysis Pipeline
How to start processing the raw imaging data generated with a Miniscope, including developing a usable pipeline and demoing the Minion pipeline.
Topics covered in this lesson
- The problem: How to get traces out of video
- Accessible jupyter interface
- Noises and backgrounds
- Kernel in image filtering
- Morphological background removal
- Motion correction
- Peak-to-noise ration refine
- Minian output
- Downstream analysis with ezTrack
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