Laura Waller - Miniscope User and Developer Talk
Laura Waller - Miniscope User and Developer Talk
"Computational imaging for miniature miniscopes" was presented by Laura Waller at the 2021 Virtual Miniscope Workshop as part of a series of talks by leading Miniscope users and developers.
Topics covered in this lesson
- Computational imaging systems
- Computational imaging pipeline
- Lenses map points to points
- Mask-based cameras multiplex
- DiffuserCam: Stick a scatterer on a sensor
- Traditional cameras take direct measurements
- Computational cameras can multiplex
- Point spread function shifts with position
- DiffuserCam forward model is a convolution
- Image reconstruction is nonlinear optimization
- Point spread function scales with depth
- Single-shot 3D is difficult
- Compressible images can be represented with less data
- Compressed sensing to the rescue!
- Neural activity tracking with flat DiffuserScope
- Random microlenses give good SNR without ambiguity
- Open-source miniature 3D microscope version
- Fourier DiffuserScope
- Resolution is more uniform
- Raw data
- Reproducible = open-source + cheap + simple hardware
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