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Andrea Giovannucci - Miniscope User and Developer Talk

Difficulty level

"Online 1-photon vs 2-photon calcium imaging data analysis: Current developments and future plans" was presented by Andrea Giovannucci at the 2021 Virtual Miniscope Workshop as part of a series of talks by leading Miniscope users and developers.

Topics covered in this lesson
  • Outline
  • 2p and 1p calcium imaging
  • Batch and online processing
  • Online experiments with fluorescence imaging
  • Requirements for cellular brain imaging analysis
  • Motion correction: Simple trick
  • Constrained NMF: Representing neurons
  • Constrained NMF: Summary
  • The background is highly contaminated
  • Parameter sharing to reduce complexity
  • Results on simulated data
  • FIOLA: Ultrafast analysis
  • Acknowledgments