#3 Hands-On Analysis Tutorial
#3 Hands-On Analysis Tutorial
This lesson will go through how to extract cells from video that has been cleaned of background noise and motion.
Topics covered in this lesson
- Pre-processing and motion correction
- Compute a max projection
- Parameters
- Max window
- Cutoff frequency
- Threshold of this peak to noise ratio
- Over merging
- Spatial matrix initialization
- Initial spatial footprint
- Update background
- Size threshold
- Spatial update
- General visualization of the result
- Temporal update
- Visualization of the dropped cells
- Reproducibility
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