Structure of synapses

Investigating the structure of synapses with electron microscopy. Speaker: Graham Knott.

By EPFL

Published Feb, 2016

Subjects Neuroimaging General neuroscience Neuroanatomy

Difficulty level Beginner

Type Video

Collections Cellular Mechanisms of Brain Function

Learning materials

External links

View the video on YouTube

Description

Topics covered:

Imaging neuronal structure with electron microscopy
Considerations for electron microscopy of biological structures
Preparing samples for electron microscopy
Chemical fixation
Mechanism of action of aldehydes
Staining and embedding brain tissue for electron microscopy
Post staining of ultra-thin sections
Transmission electron microscope
Scanning electron microscope
Sequential imaging
Block face scanning electron microscopy
Synaptic ultrastructure