Investigating the structure of synapses with electron microscopy. Speaker: Graham Knott.
Imaging neuronal structure with electron microscopy. Considerations for electron microscopy of biological structures. Preparing samples for electron microscopy. Chemical fixation. Mechanism of action of aldehydes. Staining and embedding brain tissue for electron microscopy. Post staining of ultra-thin sections. Transmission electron microscope. Scanning electron microscope. Sequential imaging. Block face scanning electron microscopy. Synaptic ultrastructure. Speaker: Graham Knott.